Research Brief
Storing Kisspeptin: Best Practices
30 Citacoes PubMedRevisado por EspecialistasCertificado GMPUltima Revisao: julho de 2026
Condições Recomendadas de Armazenamento Laboratorial
Liofilizado Powder: Store at −20°C for estabilidade a longo prazo. Proteja da luz e da umidade.
Reconstituted Solution: Kp-54 in 0.9% saline remains estavel (>90% concentration) por até 60 days at 4°C. For daily use, aliquot to minimize freeze-thaw cycles.
Intranasal Formulation: 3.5 mg/mL in 0.9% saline; store refrigerated (2–8°C) and use dentro de recommended stability window.
Handling: Standard aseptic technique. Dosing regimen matters: pulsatile = estimulacao; continuous = dessensibilizacao (HPG supressao).
Referencias
- Lee JH, Miele ME, Hicks DJ, Phillips KK, Trent JM, Weissman BE, Welch DR. KiSS-1, um(a) novo(a) human malignant melanoma metastasis-suppressor gene. Journal do(a) National Cancer Institute. 1996;88(23):1731-1737.
- Ohtaki T, Shintani Y, Honda S, et al. Metastasis suppressor gene KiSS-1 encodes peptide ligand of a G-protein-coupled receptor. Nature. 2001;411(6837):613-617.
- Kotani M, Detheux M, Vandenbogaerde A, et al. The metastasis suppressor gene KiSS-1 encodes kisspeptins, the natural ligands do(a) orphan G protein-coupled receptor GPR54. Journal of Biological Chemistry. 2001;276(37):34631-34636.
- Seminara SB, Messager S, Chatzidaki EE, et al. The GPR54 gene como um(a) regulator of puberty. New England Journal of Medicine. 2003;349(17):1614-1627.
- Dhillo WS, Chaudhri OB, Patterson M, et al. Kisspeptin-54 estimula the hypothalamic-pituitary gonadal axis in human males. Journal of Clinical Endocrinology & Metabolism. 2005;90(12):6609-6615.
- Dhillo WS, Chaudhri OB, Thompson EL, et al. Kisspeptin-54 estimula gonadotropin release a maioria potently durante o(a) preovulatory phase do(a) menstrual cycle in women. Journal of Clinical Endocrinology & Metabolism. 2007;92(10):3958-3966.
- World Anti-Doping Agency. The Lista de Substancias Proibidas. S2 Hormonios Peptidicos, Fatores de Crescimento, Substancias Relacionadas e Mimeticos. WADA. Updated 2025.
- de Roux N, Genin E, Carel JC, Matsuda F, Chaussain JL, Milgrom E. Hypogonadotropic hypogonadism due to loss of function do(a) KiSS1-derived peptide receptor GPR54. Proceedings do(a) National Academy of Sciences. 2003;100(19):10972-10976.
- Abbara A, Eng PC, Phylactou M, et al. Kisspeptin agonista do receptor has potencial terapeutico for female reproductive disorders. Journal of Clinical Investigation. 2020;130(12):6739-6753.
- Abbara A, Jayasena CN, Christopoulos G, et al. Efficacy of kisspeptin-54 to trigger oocyte maturacao in women at alto(a) risk of OHSS during IVF therapy. Journal of Clinical Endocrinology & Metabolism. 2015;100(9):3322-3331.
- Abbara A, Clarke S, Islam R, et al. A second dose of kisspeptin-54 melhora oocyte maturacao in women at alto(a) risk of OHSS: a phase 2 randomizado controlled trial. Human Reproduction. 2017;32(9):1915-1924.
- Jayasena CN, Nijher GM, Chaudhri OB, et al. Subcutaneous injection of kisspeptin-54 acutely estimula gonadotropin secretion in women with hypothalamic amenorrhea, but cronico(a) administration causes tachyphylaxis. Journal of Clinical Endocrinology & Metabolism. 2009;94(11):4315-4323.
- Jayasena CN, Nijher GM, Abbara A, et al. Twice-weekly administration of kisspeptin-54 por 8 semanas estimula release of reproductive hormones in women with hypothalamic amenorrhea. Clinical Pharmacology & Therapeutics. 2010;88(6):840-847.
- MacLean DB, Matsui H, Suri A, Neuwirth R, Colombel M. Sustained exposure para o(a) investigacional kisspeptin analog, TAK-448, down-regula testosterone no(a) castration range in healthy males e em patients with prostate cancer. Journal of Clinical Endocrinology & Metabolism. 2014;99(8):E1445-E1453.
- Skorupskaite K, et al. KP-10 infusion in PCOS women. Human Reproduction. 2020.
- Comninos AN, Wall MB, Demetriou L, et al. Kisspeptin modula sexual and emotional brain processing in humans. Journal of Clinical Investigation. 2017;127(2):709-719.
- Mills EG, et al. HSDD in men — kisspeptin aumenta penile tumescence and sexual brain processing. JAMA Network Open. 2023.
- Izarraras L, et al. Kisspeptin agonist reduz hepatico(a) de novo lipogenesis in MASLD via AMPK-SREBP-1c-CIDEA. 2025.
- Mills EG, et al. Intranasal kisspeptin-54 rapidly estimula gonadotropin release in humans: a non-invasive delivery route. eBioMedicine. 2025.
- Jayasena CN, Abbara A, et al. Kisspeptin-54 desencadeia egg maturacao in women undergoing in vitro fertilization. Journal of Clinical Investigation. 2014;124(8):3667-3677.
- Comninos AN, et al. Acute kisspeptin administration aumenta osteocalcin in healthy men. Journal of Clinical Endocrinology & Metabolism. 2022.
- Izzi-Engbeaya C, et al. Kisspeptin aumenta glucose-estimulou secrecao de insulina in healthy men. Diabetes, Obesity and Metabolism. 2018.
- Seminara SB, et al. Continuous human metastin 45-54 infusion desensitizes GPR54-induziu GnRH release in juvenile male rhesus monkeys. 2006.
- Terse PS, et al. Kisspeptin-10 toxicology studies in dogs — NOAEL at 1000 µg/kg IV × 14 days. 2021.
- Thompson EL, et al. Chronic subcutaneo(a) administration of kisspeptin-54 causes testicular degeneration in adult male rats. 2006.
- Dinh TO, et al. Kisspeptin-13 exacerbates cronico(a) doenca renal and uremic cardiomiopatia in rats. 2023.
- George JT, Veldhuis JD, Roseweir AK, et al. Kisspeptin-10 e um(a) potente stimulator of LH and aumenta pulse frequency in men. Journal of Clinical Endocrinology & Metabolism. 2011;96(8):E1228-E1236.
- Thurston L, et al. Kisspeptin modula brain activity in sexual desire regions in women with HSDD. JAMA Network Open. 2022.
- Nishizawa N, Takatsu Y, et al. Design and synthesis of TAK-448, an investigacional nonapeptide KISS1R agonist. Journal of Medicinal Chemistry. 2016;59(19):8804-8811.
- Chan YM, Butler JP, Pinnell NE, et al. Kisspeptin resets the hypothalamic GnRH clock in men. Journal of Clinical Endocrinology & Metabolism. 2011;96(6):E908-E915.
Perguntas de Pesquisa Relacionadas
APENAS PARA USO EM PESQUISA
Este conteudo e fornecido apenas para fins educacionais e informativos. Os produtos sao fornecidos apenas para estudos in vitro e nao sao medicamentos, drogas ou suplementos. Nao aprovado pela FDA para prevenir, tratar ou curar qualquer condicao.